Study of Thitonia Diversifolia Extract in Histomorphology of Pancreas and Interleukin-1beta expression on Aloxan Induced Wistar Rats

The purpose of this study is to determine effect antiinflammation Thitonia diversifolia (TD) extracts in histopathology liver and pancretic tissue on alloxan-induced Wistar rat. Thirty two male rats (Rattus norvegicus) with a body weight (bw)150-200 g 3 month age, were allocated into four groups, eight animals per group. control group received normal saline (P0), positive CMC Na 0.01% (P1), the treatment 100 mg/kg bw TD (P2) metformin (P3) respectively for 7 days. Bloods serum collected measuring blood glucose (BG). cholesterol insulin (INS) concentrations. levels concentrations analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All animal sacrified staining haematoxylin eosin. extract significantly decreased level glucoseand choelsterol compared postive (p<0.05). INS could be detected its increased Histopathological showed that induction alloxan severe multifocal degeneration, vacuolisation, cell inflammation, acute langerhan cells injury destruction cells. Immunohistochemical labeled IL-1β monoclonal antibodies (Mab) lowering expression reveal as brownish color aggregates langerhans This was concluded inhibited hepatic injuries decreasing rats.